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serca2a primary antibodies  (ABclonal Biotechnology)


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    ABclonal Biotechnology serca2a primary antibodies
    Serca2a Primary Antibodies, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serca2a primary antibodies/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    serca2a primary antibodies - by Bioz Stars, 2026-05
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    Protein expression levels and representative images. ( a ) <t>SERCA2a</t> protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.
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    serca2a primary antibodies  (ABclonal Biotechnology)
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    Protein expression levels and representative images. ( a ) <t>SERCA2a</t> protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.
    Serca2a Primary Antibodies, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Protein expression levels and representative images. ( a ) <t>SERCA2a</t> protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.
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    Protein expression levels and representative images. ( a ) <t>SERCA2a</t> protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.
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    Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, <t>SERCA2a,</t> CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.
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    Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, <t>SERCA2a,</t> CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.
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    Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, <t>SERCA2a,</t> CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.
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    Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, <t>SERCA2a,</t> CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.
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    The effect of <t>SERCA2a</t> blockade with thapsigargin. Representative example of Ca +2 levels recording (fluorescence of Fluo-5N) after adding thapsigargin to the HL-1 cardiomyocytes.
    Primary Antibody Serca2a A010 23s, supplied by Badrilla Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Protein expression levels and representative images. ( a ) SERCA2a protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.

    Journal: International Journal of Molecular Sciences

    Article Title: Sacubitril/Valsartan Combination Partially Improves Cardiac Systolic, but Not Diastolic, Function through β-AR Responsiveness in a Rat Model of Type 2 Diabetes

    doi: 10.3390/ijms251910617

    Figure Lengend Snippet: Protein expression levels and representative images. ( a ) SERCA2a protein expression level normalized to GAPDH; ( b ) The ratio of p-PLN to PLN; ( c ) The ratio of PLN to SERCA2a. C, Control ( n = 5); D, Diabetic ( n = 5); SV, Sacubitril/valsartan-treated diabetic ( n = 5); and V, Valsartan-treated diabetic ( n = 4). *, p < 0.05; **, p < 0.01 compared to control.

    Article Snippet: GAPDH (14C10) primary antibody (CST2118S) (RRID: AB_561053), PLN primary antibody (CST8495) (RRID: AB_10949105), p-PLN (PLN ser16/thr17 ) primary antibody (CST8496) (RRID: AB_10949102), SERCA2a primary antibody (CST4388) (RRID: AB_2227684), and antirabbit secondary antibody (CST7074) (RRID: AB_2099233) were obtained from Cell Signaling (Danvers, Massachusetts, USA).

    Techniques: Expressing, Control

    Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, SERCA2a, CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.

    Journal: JCI insight

    Article Title: Cardiac gene therapy treats diabetic cardiomyopathy and lowers blood glucose.

    doi: 10.1172/jci.insight.166713

    Figure Lengend Snippet: Figure 2. Exogenous cBIN1 normalizes membrane microdomains at t-tubules. (A) Representative transmission electron microscopy images of post- treatment hearts (scale bar: 1 μm) from each group (left). Quantification of the degree of contour of t-tubules (n = 100–101 t-tubules from 16–40 images of 2–3 myocardial sections and 3 hearts from each group). Data are presented as percentage of t-tubules. χ2 test was used to compare t-tubule contour between groups. *** indicates P < 0.001 for db/m + GFP versus db/db + GFP; ††† indicates P < 0.001 for db/db + GFP versus db/db + cBIN1. (B) Western blots of cBIN1, SERCA2a, CaV1.2, RyR2, GLUT4, and IRAP in total cardiac microsome and sucrose-gradient isolated TT/jSR fraction (F4) from each group (n = 5 hearts per group). (C) Representative spinning disc confocal images of posttreatment mouse myocardium with power spectrum analysis of boxed areas, and quantification of SERCA2a peak power density at t-tubules (n = 35–44 cells from 3 hearts per group). Nonparametric Kruskal-Wallis test fol- lowed by Dunn’s test was used for comparison between selected pairs. *, **, *** indicates P < 0.05, 0.01, and 0.001, respectively, for comparison versus db/m + GFP; †, ††, ††† indicates P < 0.05, 0.01, 0.001, respectively, for comparison between db/db + GFP and db/db + cBIN1.

    Article Snippet: For V5, SERCA2a, GLUT4, and IRAP labeling, permeabilized and blocked tissue sections or fixed cardiomyocytes were incubated with primary antibodies against V5 (V8317-2MC, Sigma-Aldrich), SERCA2a (ab2861, Abcam), GLUT4 (4670-1725GA, Bio-Rad Laboratories), or IRAP (6918S, Cell Signaling Technology) overnight at 4°C.

    Techniques: Membrane, Transmission Assay, Electron Microscopy, Western Blot, Isolation, Comparison

    Figure 5. AAV9-cBIN1 normalizes cardiac proteomics in diabetic mice. (A) Bar graphs of LFQ LC-MS/MS data (fold changes over control db/m mice) of SERCA2a, RyR2, GLUT4, and IRAP from the db/m mice treated with AAV9-GFP (n = 4 hearts) and db/db mice treated with AAV9-GFP (n = 4 hearts) or cBIN1 (n = 3 hearts). (B) PCA plot of all 3 groups (n = 2 repeats/heart × 3–4 hearts/group) generated based on LFQ LC-MS/MS proteomics. All data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni’s test or Kruskal-Wallis test followed by Dunn’s test was used for comparison between the selected pairs. * indicates P < 0.05 for comparison versus db/m + GFP; † indicates P < 0.05 for comparison between db/db + GFP and db/db + cBIN1.

    Journal: JCI insight

    Article Title: Cardiac gene therapy treats diabetic cardiomyopathy and lowers blood glucose.

    doi: 10.1172/jci.insight.166713

    Figure Lengend Snippet: Figure 5. AAV9-cBIN1 normalizes cardiac proteomics in diabetic mice. (A) Bar graphs of LFQ LC-MS/MS data (fold changes over control db/m mice) of SERCA2a, RyR2, GLUT4, and IRAP from the db/m mice treated with AAV9-GFP (n = 4 hearts) and db/db mice treated with AAV9-GFP (n = 4 hearts) or cBIN1 (n = 3 hearts). (B) PCA plot of all 3 groups (n = 2 repeats/heart × 3–4 hearts/group) generated based on LFQ LC-MS/MS proteomics. All data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni’s test or Kruskal-Wallis test followed by Dunn’s test was used for comparison between the selected pairs. * indicates P < 0.05 for comparison versus db/m + GFP; † indicates P < 0.05 for comparison between db/db + GFP and db/db + cBIN1.

    Article Snippet: For V5, SERCA2a, GLUT4, and IRAP labeling, permeabilized and blocked tissue sections or fixed cardiomyocytes were incubated with primary antibodies against V5 (V8317-2MC, Sigma-Aldrich), SERCA2a (ab2861, Abcam), GLUT4 (4670-1725GA, Bio-Rad Laboratories), or IRAP (6918S, Cell Signaling Technology) overnight at 4°C.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Control, Generated, Comparison

    The effect of SERCA2a blockade with thapsigargin. Representative example of Ca +2 levels recording (fluorescence of Fluo-5N) after adding thapsigargin to the HL-1 cardiomyocytes.

    Journal: Anatolian Journal of Cardiology

    Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

    doi: 10.5152/AnatolJCardiol.2022.1223

    Figure Lengend Snippet: The effect of SERCA2a blockade with thapsigargin. Representative example of Ca +2 levels recording (fluorescence of Fluo-5N) after adding thapsigargin to the HL-1 cardiomyocytes.

    Article Snippet: After blocking, the membranes were washed using Tris-buffered saline with Tween (TBS-T) and then incubated with primary antibody SERCA2a (A010-23S, Badrilla, Leeds, UK, dilution 1:1000), RyR2 (ARR-002, Alomone Labs, Jerusalem, Israel, dilution 1:1000) and β-tubulin (ab6046, Abcam, Cambridge, UK, dilution 1:1000) overnight.

    Techniques: Fluorescence

    The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).

    Journal: Anatolian Journal of Cardiology

    Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

    doi: 10.5152/AnatolJCardiol.2022.1223

    Figure Lengend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).

    Article Snippet: After blocking, the membranes were washed using Tris-buffered saline with Tween (TBS-T) and then incubated with primary antibody SERCA2a (A010-23S, Badrilla, Leeds, UK, dilution 1:1000), RyR2 (ARR-002, Alomone Labs, Jerusalem, Israel, dilution 1:1000) and β-tubulin (ab6046, Abcam, Cambridge, UK, dilution 1:1000) overnight.

    Techniques: Gene Expression, Comparison

    The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a protein expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a protein expression. n = 6. No statistically significant comparison.

    Journal: Anatolian Journal of Cardiology

    Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

    doi: 10.5152/AnatolJCardiol.2022.1223

    Figure Lengend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a protein expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a protein expression. n = 6. No statistically significant comparison.

    Article Snippet: After blocking, the membranes were washed using Tris-buffered saline with Tween (TBS-T) and then incubated with primary antibody SERCA2a (A010-23S, Badrilla, Leeds, UK, dilution 1:1000), RyR2 (ARR-002, Alomone Labs, Jerusalem, Israel, dilution 1:1000) and β-tubulin (ab6046, Abcam, Cambridge, UK, dilution 1:1000) overnight.

    Techniques: Expressing, Comparison